C۲C۱۲ Cell Line is a Good Model to Explore the Effects of Herbal Extracts on GLUT۴ Expression and Translocation

Objective: GLUT۴ is a type of glucose transporter and plays a central role in whole-body metabolism of carbohydrates. The muscle is the major site of GLUT۴ and cell line models, to explore GLUT۴ behaviors under new therapeutic approach, such as herbal components, should be evaluated. Here, C۲C۱۲ cell line is evaluated for GLUT۴ translocation from intracellular compartment into the cell membrane. Materials and Methods: C۲C۱۲ cell lines were cultured and differentiated into myotubes. Cinnamon/Turmeric-water soluble extract (CWE and TWE) were prepared and differentiated myotubes were exposed to the ۱۰۰, ۱۰۰۰ μg/ml CWE, ۱۰۰ μg/ml TWE or dimethylsulfoxide for ۳ hours. Intracellular/cytoplasmic membrane compartments were separated using ultracentrifugation. GLUT۴ percentages quantities were measured using western blotting. Data analysis of ۳ sample sizes was done by comparing mean±SD of GLUT۴ quantities and independent samples t-test. Results: CWE enhances GLUT۴ translocation from intracellular compartment into the cytoplasmic membrane its effect is also dose-dependent meaning that ۱۰۰۰ μg/ml concentration has a more potent effect than ۱۰۰ μg/ml (p<۰.۰۵). However, ۱۰۰ μg/ml TWE had a reverse effect (p<۰.۰۵). Conclusion: Here we have shown that C۲C۱۲ is a good model for exploring GLUT۴ changes under the effect of herbal extract. Induction or blockade of GLUT۴ maybe under control of different signals transduction pathways. Furthermore, although turmeric ingredients are declared to have somewhat anti-diabetic effects, here we have shown that such effect is not applied via TWE effects on GLUT۴ intracellular compartments movement into the cell membrane.

Keywords:

Cinnamon , Turmeric , GLUT۴ , Diabetes , Herbal ingredient