Metallophosphoesterases of Chlamydomonas reinhardtii and Analyses of Their Transcription Levels Under Phosphate Deficiency
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JASTMO-25-2_004
تاریخ نمایه سازی: 22 آبان 1402
Abstract:
Phosphorous (P) is an important macroelement for all organisms. However, there is a finite amount of P on Earth, therefore, new enzymes and technologies are needed for better P use in agriculture. Metallophosphoesterases are a large group of evolutionarily related proteins that are important in biotechnology. We found fourteen putative Metallophosphoesterase (MPA) genes in the genome of Chlamydomonas reinhardtii. Our RT-PCR analyses showed that some of these genes were constitutively expressed, and some were upregulated under phosphate deficiency. These results and bioinformatic analyses suggest that two of the genes (MPA۱۱ and MPA۱۳) are transcribed in high levels and the putative polypeptides are predicted to be secreted to extracellular space, making them ideal to be used in biotechnological applications. Phylogenetic analyses show that MPA۱۱ and MPA۱۳ are related to known phytases from plant species, suggesting MPA۱۱ and MPA۱۳ might have specific phytase activity. In light of these results, we discuss the potential of C. reinhardtii as a phytase producing organism for agricultural and industrial use.
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Authors
G. Hatice Havutcu
Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye.
M. Aksoy
Department of Agricultural Biotechnology, Faculty of Agriculture, Akdeniz University, Türkiye.
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