Central Effect of Exogenous Histamine on Pain Induced by Sub-Plantar Injection of Formalin in Rabbits

In the present study, the effects of intracerebroventricular (ICV) administration of normal saline (control), histamine, mepyramine (a histamine H۱-receptor antagonist) and ranitidine (a histamine H۲-receptor antagonist) were investigated on the formalin-induced pain in rabbits. Subcutaneous (SC) injection of a formalin (۱۰۰ μl, ۵%) solution into the ventral surface of the right hind paw was performed, and the time durations spent licking and biting the injected paw were measured in ۱۰ min blocks for ۱ h. The SC injection of formalin produced a short-lasting (۱۰ min) pain response. The ICV injection of histamine at doses of ۲۵, ۵۰ and ۱۰۰ μg significantly (P < ۰.۰۵) decreased the time duration spent licking and biting the injected paw. Mepyramine and ranitidine, used alone produced no effects. The ICV pretreatments with mepyramine and ranitidine at the same dose of ۲۰۰ μg significantly (P < ۰.۰۵) prevented histamine (۱۰۰ μg, ICV)-induced antinociception. These results indicate that activation of brain histamine with ICV injection of exogenous histamine produces antinociception. Central histamine H۱ and H۲ receptors may be involved in the centrally administered histamine-induced antinociception in the formalin-induced pain in rabbits.

Keywords:

brain , histamine , Formalin-induced pain , Rabbits