Molecular study on infection rates of Anaplasma ovis and Anaplasma marginale in sheep and cattle in West-Azerbaijan province, Iran
Publish place: Veterinary Research Forum، Vol: 7، Issue: 2
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_VRFAN-7-2_011
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma marginale in sheep and dairy cattle in West-Azerbaijan province, Iran. A total number of ۲۰۰ blood samples were randomly collected via the jugular vein from apparently healthy cattle (۱۰۰) and sheep (۱۰۰). The extracted DNA from blood cells was screened using genus-specific (Anaplasma spp.) nested-polymerase chain reaction (PCR) based on ۱۶S rRNA gene primer sets. Species-specific PCR was set up using major surface protein ۴ (MSP۴) gene primer set. None of cattle blood samples were positive for Anaplasma spp. by the first nested PCR. Five samples among the ۱۰۰ sheep blood samples were both positive in the first nested PCR and A. ovis -specific PCR, based on MSP۴ gene. In total, ۵.۰۰% of animals were A. ovis positive. This study identified a low prevalence of A. ovis in the blood of apparently healthy sheep in West Azerbaijan province.
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Authors
Vahid Noaman
Veterinary Research Department, Isfahan Agriculture and Natural Resources Research and Education Center, AREEO , Isfahan, Iran
Davood Bastani
Graduate Student, Faculty of Veterinary Medicine, Islamic Azad University, Boroujerd Branch, Boroujerd, Iran
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