Protective effects of vitamin E and Cornus mas fruit extract on methotrexate-induced cytotoxicity in sperms of adult mice
Publish place: Veterinary Research Forum، Vol: 5، Issue: 1
Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_VRFAN-5-1_004
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
This study was aimed to assess the protective effects of Cornus mas fruit extract (CMFE) and vitamin E (Vit E) on sperm quality parameters in the methotrexate (MTX)-treated mice. Forty-eight young adult male mice (۸-۱۲ weeks) were randomly divided into six groups including control and test groups. The control group received normal saline orally , and the test groups were treated MTX (۲۰ mg kg-۱, ip, once weekly), MTX + CMFE (۲۵۰ mg kg-۱), MTX + CMFE (۵۰۰ mg kg-۱), MTX + CMFE (۱۰۰۰ mg kg-۱), and MTX + Vit E (۱۰۰ IU kg-۱, po) for ۳۵ consecutive days. On day ۳۵, after euthanasia the epididymal sperms were isolated. Then the total mean sperm count, sperm viability and motility were determined. The total antioxidant capacity (TAOC) of all experimental groups were also evaluated. The MTX-treated animals showed a significant changes in all parameters of sperm quality assessment compared to the control group. Both Vit E and CMFE were able to protect from MTX-induced effects on sperm maturity and DNA damage. Co-administration of MTX and CMFE and/or Vit E resulted in protection from MTX-reduced TAOC. In conclusion, these data suggested that MTX administration could adversely affect the sperm quality. Moreover, the protective effect of Vit E and CMFE on MTX-induced sperm toxicity was also documented.
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Authors
Leila Zarei
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Rajabali Sadrkhanlou
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Rasoul Shahrooz
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Hassan Malekinejad
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Behroz Eilkhanizadeh
Department of Pathology, Faculty of Medicine, University of Medical Sciences, Urmia, Iran
Abbas Ahmadi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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