Immunohistochemical investigation of parainfluenza ۳ virus in sheep pneumonia

The aim of this study was to determine the prevalence of parainfluenza ۳ (PI۳) virus antigen through histopathological and immunohistochemical (IHC) methods in sheep lung samples collected from Erzurum province, Türkiye. Between August and November ۲۰۱۷, ۱۴۶۲ sheep were dissected in the slaughterhouse and their lungs were examined macroscopically. In total, ۱۰۰ of the lung samples with pneumonia were selected. Routine histopathological and IHC analyses of the collected lung tissues with pneumonia were performed. Pneumonia observed through macroscopical and histopathological examinations of the lung samples was classified as purulent-catarrhal bronchopneumonia (۱۴.۰۰%), fibrinous bronchopneumonia (۲۳.۰۰%), interstitial pneumonia (۶۹.۰۰%), granulomatous pneumonia (۷.۰۰%), verminous pneumonia (۱۹.۰۰%) and pulmonary adenomatosis (۶.۰۰%). Two or three types of pneumonia were observed in many of the same cases. The PI۳ virus antigen positivity rate in the IHC analysis of sheep lung samples was ۱۹.۰۰%. In the IHC tracing, positivities were found mostly in the alveolar macrophages and cytoplasm of bronchial, bronchiolar and alveolar epithelial cells. As a result, the prevalence of PI۳ virus in sheep in Erzurum province, Türkiye, was determined to be ۱۹.۰۰% using KLN BVB IHC method.

Keywords:

Histopathology , Immunohistochemistry , parainfluenza ۳ , pneumonia , Sheep