Irradiated oocysts in combination with inulin adjuvant-induced potent immune responses against Eimeria tenella infection in broiler chickens
Publish place: Veterinary Research Forum، Vol: 14، Issue: 8
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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JR_VRFAN-14-8_003
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
Coccidiosis is the leading parasitic disease in poultry. One of the most critical Eimeria species, Eimeria tenella, lives inside the cecal epithelial cells and induces bloody coccidiosis. The present study evaluated the effect of radiation-attenuated E. tenella oocytes mixed with inulin adjuvant on broiler chicken. Initially, the effect of irradiation on oocyst attenuation was confirmed. Then, one-day-old broilers (n = ۹۰) were divided into nine groups on seven days of age as follow: Group ۱ (۴۰۰ attenuated oocysts + ۱.۰۰ mg of adjuvant), group ۲ (۴۰۰ attenuated oocysts + ۰.۵۰ mg adjuvant), group ۳ (۲۰۰ attenuated oocysts + ۱.۰۰ mg of adjuvant), group ۴ (۲۰۰ attenuated oocysts + ۰.۵۰ mg adjuvant), group ۵ (۱.۰۰ mg adjuvant), group ۶ (۴۰۰ attenuated oocysts), group ۷ (commercial vaccine), group ۸ (negative control) and group ۹ (blank). On day ۲۱, we performed a challenge with E. tenella oocytes and investigated oocyst output and average weekly weight throughout the study. At the end of the study, we evaluated macroscopic lesion, histology, cytokine level and leukogram status. The results showed a statistically significant difference among groups. Furthermore, the optimal dose was ۴۰۰ irradiated oocysts and ۱.۰۰ mg of inulin. Moreover, an X-ray could reduce the virulence of E. tenella oocytes. Inulin alone or combined with attenuated oocysts showed an acceptable effect on evaluated parameters.
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Authors
Monireh Falsafi
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Nowruz Delirezh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Elham Safarzadeh
Department of Medical Microbiology, Parasitology and Immunology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
Alireza Talebi
Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Zahra Heidari
Department of Medical Microbiology, Parasitology and Immunology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran
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