Probing into the Network of Transcription Factor Twist-Related Protein ۱ (TWIST۱) in Breast Cancer Metastasis
Publish place: Middle East Journal of Cancer، Vol: 14، Issue: 1
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
View: 66
This Paper With 12 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_MISJ-14-1_005
تاریخ نمایه سازی: 25 آبان 1402
Abstract:
Background: The transcription factor twist-related protein ۱ (TWIST۱) plays a major role in the prognosis of breast cancer. Our present study aimed to identify the network of TWIST۱ with related oncogenes and their associated miRNAs.Method: This in silico study included the differential expression analysis of genes and miRNA associated with breast carcinoma. The breast cancer patients’ data were retrieved from the Gene Expression Omnibus database and the differential expression analysis was done using GEO۲R. Transfac analysis was performed to determine the binding sites of TWIST۱. We predicted the target genes of MicroRNA-۹۶ (miR-۹۶) using miRBase. An integrated network was generated among TWIST۱ and target genes of miR-۹۶ through Gene MANIA. Survival analysis was carried out for TWIST۱ using UALCAN. Experimental methods, including gene expression analysis, were performed in the MDA-MB-۲۳۱ cell line for validating in silico findings.Results: miR-۹۶, the second differentially expressed miRNA among the top ۲۵۰ miRNAs, was found to have eight binding sites for TWIST۱. TWIST۱ was observed to be significantly correlated with patient prognosis. ACTN۴, BCL۲, and FRMD۴A were upregulated and CAMTA۱, DAB۲IP, and E- Cadherin were downregulated in the expression studies carried out in the MDA-MB-۲۳۱ breast cancer cell line.Conclusion: A network between TWIST۱ and target genes of miR-۹۶ was analyzed. Hence, targeting the genes linked with miR-۹۶ could work toward an efficient therapeutic option for breast cancer metastasis.
Keywords:
Twist-related protein ۱ , Gene expression , Omnibus datasets , MicroRNA-۹۶ , Survival analysis , MDA-MB-۲۳۱ breast cancer cells
Authors
Jeevitha Priya Manoharan
Department of Biotechnology, PSG College of Technology, Coimbatore-۶۴۱۰۰۴, Tamil Nadu, India
Kavinkumar Nirmala Karunakaran
Department of Biotechnology, PSG College of Technology, Coimbatore-۶۴۱۰۰۴, Tamil Nadu, India
Gomathi Dasarathan
Department of Biotechnology, PSG College of Technology, Coimbatore-۶۴۱۰۰۴, Tamil Nadu, India
Subramanian Vidyalakshmi
Department of Biotechnology, PSG College of Technology, Coimbatore-۶۴۱۰۰۴, Tamil Nadu, India
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :