Effects of explant type and growth regulators on callus induction in four ecotypes of Persian shallot
Publish place: Biotechnology of Medicinal Plants، Vol: 5، Issue: 1
Publish Year: 1398
نوع سند: مقاله ژورنالی
زبان: Persian
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شناسه ملی سند علمی:
JR_JMPBZ-5-1_005
تاریخ نمایه سازی: 27 آبان 1402
Abstract:
Allium hirtifolium commonly known as Persian shallot is an important wild medicinal plant distributed from North West to central and South West of Iran. To establish an efficient protocol for callus induction, the effects of explant type and growth regulators on callus induction in four ecotypes of Persian shallot were evaluated. Two explants types included basal plates and young leaves were cultured on MS media supplemented with ۱.۵ mg l-۱ of ۲,۴-dichlorophenoxyacetic acid (۲,۴-D) or ۱.۵ mg l-۱ of ۱-naphthalene acetic acid (NAA) in combination with ۰, ۰.۵ or ۱ mg l-۱ of ۶-benzylaminopurine (BAP). All the cultures were maintained at ۲۵±۱°C in the dark. The results showed that basal plate was the best explant for callus induction when cultured on medium supplemented with ۱.۵ mg l-۱ ۲,۴-D and ۰.۵ mg l-۱ BAP. This optimized protocol will be useful for any future breeding improvement programs of Persian shallot using biotechnological means.
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Authors
Nasrin Farhadi
Department of Horticulture, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Jaber Panhandeh
Department of Horticulture, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Bahram Sanati Monfared
Expert of Standard General Office of Zanjan Province, Zanjan, Iran