Genetic Diversity and Structure of Iranian Teucrium (Teucrium polium L.) Populations Assessed by ISSR Markers

Understanding of genetic diversity is essential in breeding programs and plant genetic resources management. In this study, the genetic diversity of ۷۷ individuals of Teucrium from different regions of Iran was investigated using ۱۸ ISSR markers. A total number of ۱۹۸ bands were detected by ISSR primers, of which ۱۸۴ (۹۲.۹%) bands with an average of ۱۰.۲ bands per primer were polymorphic. The Percentage of Polymorphic bands (PPL) ranged from ۸۰ (UBC۸۳۴) to ۱۰۰% (UBC۸۱۱, ۸۱۲, ۸۱۸, ۸۲۰, ۸۲۵, ۸۲۶, and UBC۸۵۵). The average Polymorphic Information Content (PIC), Shannon’s Information index (I), and Number of effective alleles (Ne) were ۰.۳۹, ۰.۵۲۶, and ۱.۶, respectively. The Analysis of Molecular Variance (AMOVA) revealed the higher level of genetic variation within populations (۷۷%) compared to among populations (۲۳%). Cluster analysis separated the individuals into three major groups using WPGMA based on Nei’s genetic distance coefficients. In addition, a model-based Bayesian approach subdivided the individuals into three major subgroups. The results of this study revealed that estimation of population genetics parameters using ISSR markers can be applied for assessing the differences between Teucrium populations and management of the genetic resources.

Keywords:

AMOVA , Model-based cluster , Shannon's information index , Teucrium polium L