Assessing the genetic diversity of ۸۹ flue-cured tobacco varieties using morphological traits and inter-simple sequence repeat markers

The genetic diversity of ۸۹ flue-cured tobacco varieties was examined using ۱۲ ISSR primers. These cultivarswere evaluated at the Guilan Tobacco Research Center, Rasht, Iran, using a ۷×۷ simple lattice design with two replications, and ۱۲ morphological traits. The total number of PCR amplified products was ۱۴۳ bands ranging from ۴۵۰ to ۳۰۰۰ bp, of which ۱۰۸ bands (۷۴.۲۸%) were polymorphic. Primers UBC ۸۱۱ and UBC ۸۱۴ with ۱۶ bands and UBC ۸۲۵ with ۶ bands generated the highest and lowest number of bands, respectively. Of all the primers, UBC۸۱۷, UBC۸۲۴ and UBC۸۷۳ showed the maximum amount (۰.۴۷) of polymorphism information content (PIC) and the greatest diversity. To determine the genetic relationship among tobacco cultivars, cluster analysis was performed based on either morphological traits or ISSR markers using the un-weighted pair-group method with arithmetic average (UPGMA). Tobacco genotypes were divided into five main groups. Principal coordinate analysis (PCoA) on a similarity matrix of genotypes showed that the first ۱۲ coordinates explained ۶۰.۱۶% of the total variance, whereas the first two coordinates explained only ۲۸.۹۶% of total variance. Cluster analysis of morphological traits divided tobacco genotypes into five groups. Based on canonical discriminate function analysis using the Fisherlinear method, the UPGMA method separated the genotypes with ۷۸.۵% accuracy. UBC۸۱۷, UBC۸۲۴ and UBC۸۷۳ were the most informative primers and thus could be used to assess the diversity of tobacco cultivars. In addition, UBC۸۱۳, UBC۸۲۳ and UBC۸۲۶ would be appropriate ISSR primers because of the reasonable amount of PIC, Nei and Shannon’s information index.

Keywords:

bootstrap analysis , cluster analysis , Genetic relationship , molecular marker , Shannon’s information index