In-vitro speciation of molybdenum (II, VI) in human biological samples based on thiol-functionalized mesoporous silica nanoparticles and hexyl-methylimidazolium trispentafluoroethyl- trifluorophosphate
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نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_AMECJ-3-3_005
تاریخ نمایه سازی: 14 آذر 1402
Abstract:
Molybdenum (Mo) ions enter to human body from the diet or
drinking waters and have a potentially toxic effect on human. The
thiol-functionalized mesoporous silica nanoparticles (HS-MSNPs)
was used for determination and speciation of Mo (II, VI) in human
biological samples by dispersive ionic liquid-micro-solid phase
extraction (DIL-μ-SPE) coupled to electrothermal atomic absorption
spectrometry (ET-AAS). Firstly, the mixture of HS-MSNPs (۱۵
mg), the hydrophobic ionic liquid (۱-Hexyl-۳-methylimidazolium
tris(pentafluoroethyl) trifluorophosphate; [HMIM][T(PFE)PF۳]) and
acetone injected to ۱۰ mL of human blood and serum samples. After
shaking for ۵ min, the Mo(II) and Mo(VI) ions were extracted with
thiol group of MSNPs at pH ۶ and ۲, respectively and collected through
IL in bottom of conical tube by centrifuging. Then, the Mo(II,VI) ions
were back-extracted from HS-MSNPs with elent based on changing
pH, and remained solutions were determined by ET-AAS after
dilution with DW up to ۰.۵ mL, separately. So, the total of Mo(TMo)
was simply calculated by the summation of Mo(II) and Mo(VI)
content. In optimized conditions, the linear range (LR), the limit of
detection and enrichment factor (EF) for Mo(II) and Mo(VI) were
obtained (۰.۴۱-۳.۸۲ μg L−۱; ۰.۴۸–۴.۵۵ μg L−۱), (۰.۱ μg L−۱; ۰.۱۲ μg
L−۱) and (۱۹.۶; ۱۶.۵) for ۱۰ mL of human blood samples, respectively
(Mean of RSD%=۳.۳). At optimized pH, the adsorption capacities of
the HS-MSNPs for Mo(II) and Mo(VI) was obtained ۶۸.۷ mg g-۱ and
۵۵.۸ mg g-۱, respectively. In purposed study, a new analytical method
for rapid speciation and determination of trace amount of Mo (II, VI)
was used in human blood and serum samples. The developed method
was successfully validated by ICP-MS analysis.
Keywords:
Molybdenum , Speciation , Human serum , Ionic liquid , Thiol-functionalized bimodal mesoporous silica nanoparticles , Dispersive-ionic liquid-micro-solid phase extraction
Authors
Roya Ashouri
Department of Environmental science, Science and Research Branch, Islamic Azad University, Tehran, Iran- Department of Environmental Engineering, Faculty of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, I
Seyed Alirza Hajiseyed Mirzahosseini
Department of Environmental Engineering, Faculty of Natural Resources and Environment, Science and Research Branch, Islamic Azad University, Tehran, Iran