Protective effect of aqueous fruit extract of Mondia whitei against cadmium-induced hepatotoxicity in rats

Introduction: Mondia whitei (Hook.f.) Skeels is rich in antioxidant activity and is known for its nutritional and medicinal uses. This study evaluated the protective effect of M. whitei fruit against cadmium-induced hepatic damage in rats. Methods: Twenty-five albino (Wistar strain) rats were randomly assigned into five equal groups. Rats in group I served as control, rats in group II were intoxicated with ۵ mg/kg body weight (b.w.) cadmium chloride (CdCl۲) for ۵ days via an oral route, while groups III, IV, and V were respectively administered with ۵ mg/kg b.w. CdCl۲ for ۵ days co-treated with ۷۰ mg/kg b.w silymarin, ۲۵۰ and ۵۰۰ mg/kg b.w. of aqueous fruit extract of M. whitei (AEMW) for ۷ days. Results: Cadmium caused a significant (P < ۰.۰۵) increase in the concentration of cadmium in the liver as well as liver function markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and bilirubin. In addition, a significant (P < ۰.۰۵) elevation in the level of malondialdehyde (MDA) and a reduction in the nitric oxide (NO) and antioxidant status were noted in the CdCl۲-exposed rats; hepatic degeneration and congested portal area were also noted. These changes were, however, reduced in the cadmium-intoxicated rats co-treated with silymarin, ۲۵۰ mg/kg or ۵۰۰ mg/kg AEMW. Conclusion: Our result suggests that AEMW exerts protective effects against CdCl۲-induced hepatic damage in rats, and this might be due to the presence of phytochemicals in the plant capable of scavenging oxidative stress caused by cadmium.

Keywords:

Alanine transaminase , Liver damage , Malondialdehyde , Oxidative stress , Phytochemical