Molecular epidemiology of clinically relevant single and mixed species in a Malaysian tertiary care hospital

Background and purpose: The increasing rate of opportunistic infections caused by Candida and other yeasts is becoming a major health concern worldwide. However,systematic data on the epidemiology and the yeast species infections in Malaysia is still limited. In this regard, the present research aimed to identify pathogenic yeasts utilizing an economically practical and easily available molecular technique and evaluate the prevalence of pathogenic yeasts in a Malaysian tertiary care hospital.Materials and Methods: Yeast isolates were collected from Sultan Abdul Halim Hospital, Kedah, Malaysia, from October ۲۰۲۰ to October ۲۰۲۱. Molecular identification of the isolates was performed by one enzyme-based polymerase chain reaction restriction fragment length polymorphism method.Results: Candida albicans was the most prevalent species, accounting for ۱۲۰ isolates (۵۹%) in total. The most prevalent non albicans Candida species were C. tropicalis (n=۳۳,۱۶%), C. krusei (Pichia kudriavzevii) (n=۱۲, ۵.۸%), C. glabrata (n=۱۲, ۵.۸%), and C.parapsilosis (n=۶, ۳%). Other unusual Candida species were C. guilliermondii (۲), C.metapsilosis (۲), C. orthopsilosis (۱), C. lusitaniae (۱), C. rugosa (۱), C. haemulonii (۱), C. bracarensis (۱), and C. dubliniensis (۱). Moreover, Talaromyces marneffei (۱), Kodamaea ohmeri (۱), Cryptococcus neoformans (۳), and Cryptococcus laurentii (۱) were among the other yeasts identified.Conclusion: The Molecular technique used in this study identified ۹۶% of isolates,including mixed species. According to the findings, the most prevalent species are C. albicans, C. tropicalis, C. krusei, and C. glabrata.

Keywords:

PCR-RFLP , Msp۱ , Candida , Mixed yeast infections