Comparative and Combined Effects of Echium amoenum and Cichorium intybus Extracts on the Live of Wista Rats

The study is aimed to evaluate the effects of Echium amoenum and Cichorium intybus extracts on the liver of Wistar rats. Ninty rats were divided into ۱۵ groups. Group ۱ received physiological serum, groups ۲, ۳, ۴ and ۵ ۴۰ mg/Kg, ۶, ۷, ۸ and ۹, ۴۰۰ mg/Kg, ۱۰, ۱۱, ۱۲ and ۱۳, ۱۰۰۰ mg/Kg aqueous and ethanolic extract of C.intybus and E. amoenum, group ۱۴ and ۱۵, ۴۰۰ mg/Kg combined aqueous and ethanolic extracts. Blood samples were collected to assay AST, ALT, ALP, GGT, MDA, total protein and bilirubin levels. The effect of different concentrations of aqueous extracts of chicory and echium on AST, ALT, ALP, GGT and bilirubin levels is the same. Concentrations of ۴۰ to ۱۰۰۰ mg/kg of chicory ethanol extract had different effects on AST, ALP and bilirubin, but in the case of ALT, the differences were between ۴۰ and ۴۰۰ mg/kg. The flavonoid and anthocyanin contents were the highest in ethanolic extract of echium. Antioxidant activity and total phenols content in ethanolic extract were higher than aqueous extract. The use of aqueous extract of chicory in high concentrations did not harm the liver. Ethanolic extracts of both plants at ۱۰۰۰ mg/Kg will damage the liver. The ethanolic extracts of both plants contain many phenolic compounds that increase the inhibition of free radicals in this extract.

Keywords:

Cichorium intybus , Echium amoneum , Gamma-glutamyl transferase , malondialdehyde , Anthocyanin , flavonoid