Laboratory experiment of the extraction of active pharmaceutical ingredients of Oliveria decumbens plant by supercritical method using the ultrasound process

In this research, the Oliveria decumbens native and medicinal plant has been used. The extract of this plant contains anti-inflammatory compounds, very strong antioxidants, Allergic reduction compounds and antiviral and anticancer compounds. Extraction of this plant was investigated using supercritical extraction system using supercritical CO2 fluid in two stages with ultrasound waves and without ultrasound waves, which the research showed that the highest percentage of effective compounds was extracted by supercritical fluid through the ultrasound process. In this study, the extractive effect of two compounds of Octanoic acid and Nonadecanoic Acid as two compounds with the highest drug effect was investigated due to the anticancer and antiviral properties. This amount for the active substance Octanoic Acid in SCF state is 0.02 and in the USCF is 0.14. and for Nonadecanoic Acid in the SCF state is 0.11 and in the USCF state it is 0.86. Also, the extraction in the supercritical device was investigated in both states with ultrasound and without it, at pressures of 190, 170 and 210 bars and the temperatures of 55,45 and 65 Centigrade and frequencies of 37 and 80 kHz. The results showed that the percentage of product (gram of active ingredient / gram of sample) at the pressure of 210 bar and 37 kHz frequency had the highest extraction rate.

Laboratory experiment of the extraction of active pharmaceutical ingredients of Oliveria decumbens plant by supercritical method using the ultrasound process Keywords:

Extraction , Supercritical fluid , Ultrasound , active ingredient , Oliveria decumbens