Laboratory experiment of the extraction of active pharmaceutical ingredients of Oliveria decumbens plant by supercritical method using the ultrasound process

In this research, the Oliveria decumbens native and medicinal plant has been used. The extract of this plant contains anti-inflammatory compounds, very strong antioxidants, Allergic reduction compounds and antiviral and anticancer compounds. Extraction of this plant was investigated using supercritical extraction system using supercritical CO۲ fluid in two stages with ultrasound waves and without ultrasound waves, which the research showed that the highest percentage of effective compounds was extracted by supercritical fluid through the ultrasound process. In this study, the extractive effect of two compounds of Octanoic acid and Nonadecanoic Acid as two compounds with the highest drug effect was investigated due to the anticancer and antiviral properties. This amount for the active substance Octanoic Acid in SCF state is ۰.۰۲ and in the USCF is ۰.۱۴. and for Nonadecanoic Acid in the SCF state is ۰.۱۱ and in the USCF state it is ۰.۸۶. Also, the extraction in the supercritical device was investigated in both states with ultrasound and without it, at pressures of ۱۹۰, ۱۷۰ and ۲۱۰ bars and the temperatures of ۵۵,۴۵ and ۶۵ Centigrade and frequencies of ۳۷ and ۸۰ kHz. The results showed that the percentage of product (gram of active ingredient / gram of sample) at the pressure of ۲۱۰ bar and ۳۷ kHz frequency had the highest extraction rate.

Keywords:

Extraction , Supercritical fluid , Ultrasound , active ingredient , Oliveria decumbens