The Effectiveness of Biotin (Vitamin B۷) Added to the Diet in Improving the Efficiency of Productivity, and Some Physiological Traits for Broiler Chickens (Ross-۳۰۸) Exposed to Oxidative Stress

Biotin (B۷) acts as an antioxidant, as it inhibits the effect of many free radicals that are naturally formed within the organism's body. This study aimed to determine the effect of adding different concentrations of biotin to the diet of broilers exposed to oxidative stress in improving productive and physiological performance. ۱۸۰ unsexed Ross-۳۰۸ one-day age chicks of broiler chickens were used, and they were reared together until ۷ days. Then the chicks were randomly distributed into ۵ treatments; each treatment included ۳ replicates, ۱۲ chicks for each replicate. Oxidative stress was induced by adding ۰.۵% H۲O۲ to drinking water. The first treatment, T۱ (control), was free from adding B۷ or H۲O۲, and the second treatment (T۲): was a positive control treatment free from adding vitamin B۷ + water added to it H۲O۲ at an average of ۰.۵%. The third treatment (T۳): adding ۵۵۰ micrograms of B۷/kg of feed + water added H۲O۲ at an average of ۰.۵%. Fourth treatment (T۴): ۶۵۰ micrograms/kg vitamin B۷ + water with ۰.۵% H۲O۲ added on average. Fifth treatment (T۵): ۷۵۰ micrograms of vitamin B۷/kg of feed + ۰.۵% H۲O۲ added to the water. The results of adding vitamin B۷ to the diet of T۴ and T۵ birds exposed to oxidative stress showed a significant increase (P<۰.۰۵) in the average live body weight, total weight gain and cumulative feed consumption average, and the best cumulative feed conversion ratio for treated birds (T۳, T۴, T۵). In comparison to the other therapies, all additional vitamin B۷ treatments demonstrated a significant decrease (P<۰.۰۵) in the concentrations of glucose, cholesterol, triglycerides, as well as Malondialdehyde (MDA), and low-density lipoproteins (LDL) and a significant increase (P<۰.۰۵) in the concentrations of high-density lipoproteins (HDL) and total protein. The concentration of glutathione peroxidase (GSH-Px) was significantly elevated (P<۰.۰۵) in the serum of T۵-treated birds compared to all other treatments. We conclude that adding vitamin B۷ in different concentrations improved the productivity and some physiological traits of broilers exposed to the induced oxidative stress compared with the negative and positive control treatments.

Keywords:

Biotin , vitamin B۷ , physiological traits , Broiler Chickens , Ross-۳۰۸ , oxidative stress