Molecular Interplay of John Cunningham Virus with Interleukin ۱ Beta in Colorectal Carcinomatous Tissues from a Group of Iraqi Patients

Colorectal cancer is ranked to have high mortality among most malignancies worldwide. In the adult population, the seroprevalence rates of the John Cunningham virus (JCV) range from ۷۰% to ۹۰%. Recently the association for JCV in many malignant tumours have been reported worldwide, including colonic and rectum cancers. Interleukin-۱β (IL-۱β) can promote tumour growth where it is abundant in the tumour microenvironment, and its up-regulation is considered a poor prognostic feature in different types of solid tumours, including colon malignancies. One hundred tissue biopsies belonged to ۵۰ patients with colorectal cancers and ۳۰ benign colonic tumour patients, and ۲۰ colorectal control tissues were enrolled in this study. JCV was detected via chromogenic in situ hybridization (CISH), while IL-۱ beta was detected by immunohistochemistry (IHC). The recorded data showed that ۲۱ out of ۵۰ (۴۲%) tissue samples with colorectal carcinoma showed positive CISH reactions for JCV DNA in this study. The benign colorectal tumours group revealed positive signals in ۲ out of ۳۰ tissues representing ۶.۷% of this group. Lastly, no control tissues showed positive signals for the JCV -CISH test. The positive signals of IL-۱ Beta-IHC detection were found in ۲۶ out of ۵۰ (۵۲ %) colorectal carcinoma tissues, while in the benign colorectal tumour was ۴۳.۳% (۱۳ out of ۳۰) and in AHC was ۲۰% (۴ out of ۲۰ tissues). The high rates of JCV infection in this group of Iraqi patients with colonic adenocarcinoma in concordance with IL-۱ Beta expression could play an essential role in the development and progression of these malignant tumours along with benign colonic tumours. To analyze the concordant expression of IL-۱ beta gene and JCV in issues from a group of Iraqi patients with colonic adenocarcinomas.

Keywords:

In situ hybridization , IL۱ beta , immunohistochemistry