Genetic Detection of Genes Encodes Some Enzymes in Entamoebahistolytica in Diarrhea Children in Iraq

Entamoebahistolytica is a protozoan, an anaerobic intestinal parasite that causes about ۵۰ million infections and a mortality rate of more than ۱۰۰,۰۰۰ worldwide. For diagnosis, two hundred samples of children with diarrhea signs were evaluated using staining and polymerase chain reaction techniques. The current study recorded ۱۱ positive cases of E. histolytica, which were diagnosed by polymerase chain reaction (PCR) out of a total of ۵۱ positive cases diagnosed microscopically for pediatric children arriving at Tikrit General Hospital in Tikrit city and the nearby areas. The percentage of positive cases reached ۲۱.۵۷% for the PCR assay, as significant differences appeared compared to the microscopic examination. The results showed that the parasite infection rates differed between males (۵۴.۹%) and females (۴۵.۱%). The percentage of infected numbers in the age group less than one year was about ۴۳.۱%, while the percentage of disease control and prevention programs f infected people in the age group ۱-۲ years was (۳۱.۴%). The results showed that the percentage of infected age group between (۲-۳) years was ۱۵.۷%. The recorded data showed that ۵.۹% and ۳.۹% of the participants were infected in the age group of۳-۴ and over four years old, respectively. The genes encoded in Cysteine proteinase five and Phospholipase were diagnosed using the PCR technique. The concordance with the current study isolate and ۹۰% match globally. In conclusion, the methods of detection of E. histolytica appeared differences in positive results for this parasite.

Keywords:

Entamoebahistolytica , Cysteine proteinase ۵ , Phospholipase