Investigation of Antioxidant and Cytotoxicity Effects of Silver Nanoparticles Produced by Biosynthesis Using Lactobacillus gasseri
Publish place: Archives of Razi Institute journal، Vol: 76، Issue: 4
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ARCHRAZI-76-4_008
تاریخ نمایه سازی: 6 دی 1402
Abstract:
The biosynthesis of silver nanoparticles (AgNPs) is a new approach in nanotechnology which was optimistically implemented in medicine, food control, and pharmacology. The present study aimed to investigate the antioxidant and cytotoxicity effects of AgNPs produced by Lactobacillus gasseri filtrate. Also, changing color from yellow to brown confirmed the production of AgNPs. AgNPs were characterized using ultraviolet-visible spectroscopy, FE-SEM, and Fourier Transform Infrared Spectroscopy (FTIR). The antioxidant activity of AgNPs was tested using the DPPH assay. The scavenging test for DPPH showed ۱۹.۳%, ۳۲.۶%, ۴۷.۶%, ۷۲%, ۸۵.۳% at concentrations (۶.۲۵, ۱۲.۵, ۲۵, ۵۲, ۱۰۰) µg/ml, respectively, which proved that the scavenging percentage increased with increasing concentration. The effect of AgNPs on the chromosomal pattern was also studied. The results of the experiment of AgNPs against SK-GT-۴ cancer cells showed the toxic activity of the used particles against the strains of these human esophageal cancer cells and failed to affect normal cells.
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Authors
R Abduladheem Jabbar
Departments of Applied Sciences, University of Technology, Iraq
N Neima Hussien
Departments of Applied Sciences, University of Technology, Iraq
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