Efficacy of CpG-ODN Administration Routes on Humoral Responses against Newcastle disease in Broilers

Un-methylated cytosine-phosphate-guanosine oligodeoxynucleotides (CpG-ODN) has been considered as a powerful vaccine adjuvant and recognition of CpG-ODN by chicken leukocytes promotes their ability to fight against infections. In our study, efficacy of different routes of CpG-ODN application as an adjuvant on immune responses (antibody titer together with leukogram) following vaccination against Newcastle disease (ND) has been evaluated in broiler chickens (Ross-۳۰۸). The results indicated that routes of CpG-ODN administration influence immune responses and comparison effectiveness of CpG-OND delivery routes showed that group vaccinated by eye-drop application had the highest antibody titer than that of the group injected intramuscularly (im) and the difference was significant (p = ۰.۰۴) on day ۳۵ of age. Antibody titer of the group treated with Clone ۳۰ plus CpG-ODN via eye-drop route was higher than that of the group vaccinated with clone ۳۰ alone on days ۲۸ and ۳۵ of age and the difference was significant (p = ۰.۰۴). Co-administration of both vaccine and CpG improved outcome of leukogram of the chickens on days ۲۱ to ۴۲ of age and among the treated groups, WBC of the group received both vaccine and CpG by eye-drop route significantly (p < ۰.۰۵) differed from that of the group vaccinated with clone ۳۰ alone on days ۲۸ and ۳۵ but not on day ۴۲ of age. Average final body weight of the control group did not significantly differ from those of the treated groups at end of the experiment. In conclusion, co-administration of ND vaccine plus CpG-ODN via eye-drop route improves immune responses.

Keywords:

CpG , Antibody titer , ND , broilers , Vaccine administration route