Detection of verotoxin (Shiga-like toxin)-producing and eae harboring Escherichia coli in some wild captive and domestic Equidae and Canidae

The aim of this study was to investigate the prevalence of STEC and EPEC strains and E. coli O۱۵۷ serogroup in some Equidae and Canidae. The fecal samples of ۷۹ animals from ۶ different species were evaluated for presence of these strains. All the Isolates were tested for virulence genes using multiplex-PCR. Non-sorbitol fermenting (NSF) Escherichia coli isolates and positive strains for virulence factors were subjected to serogroupe specific PCR for rfb O۱۵۷ gene. None of the STEC, EPEC and NFS strains in this study belonged to O۱۵۷ serogroupe. While ۳۶.۶۴% of animals carried strains positive for one or more of the virulence factors tested, and ۱۸.۹% of animals harbored STEC strains (stx۱), stx۲ was not detected in this study. eae and Ehly positive strains were found in ۳.۷۹% and ۲۲.۷% of animals respectively. In conclusion, these species can act as a reservoir for EPEC and STEC strains. Also, since the study was conducted in some parts of Iran, a more accurate conclusion needs more distributed sampling. To our knowledge this is the first study which reports the faecal shedding of STEC and EPEC from wild captive Canidae and Equidae in Iran.

Keywords:

Canidae , Equidae , STEC , EPEC , Iran