Amplification, cloning and expression of Brucella melitensis bp۲۶ gene (OMP۲۸) isolated from Markazi province (Iran) and purification of Bp۲۶ Protein

Brucellosis is a debilitative disease that imposes costs on both economy and society. It is shown that although the vaccine can prevent abortion, it does not provide complete protection against infection. In Iran, Brucella melitensis is a common causative agent for brucellosis and BP۲۶ protein of this bacterium having a good antigenesity and an important vaccine candidate. In this study B. melitensis bp۲۶ gene was cloned first in to PTZ۵۷R/T vector and accessed on the PET۲۸a vector and sequenced. Recombinant vector transformed and expressed in to E. coli BL۲۱ (DE۳) and then recombinant protein was purified with Ni-NTA column of chromatography against His tag. Obtained rOmp۲۸ could be used as a research experimental tool to find its potential as a detection kit and vaccine candidate.

Keywords:

Brucella melitensis , bp۲۶ gene , OMP۲۸ , Brucellosis , Cloning , experssion