Rapid and accurate diagnosis of Foot-and-mouth disease virus by Real-time PCR in field samples

During ۲۰۱۰-۲۰۱۱, Real-time PCR procedure was used to detecting FMDV RNA on ۱۴۷ epithelium samples from the field. In this survey, for Real-time PCR from ۳D gene segment as conserve region selected for tracking all of seven serotypes FMDV. The assay detected the viral RNA in all serotypes of FMDV. The rRT-PCR specifically detected FMD virus in sample with greater sensitivity than our conventional RT-PCR procedure and our routine diagnostic procedures. Sensitivity of this technique was estimated by ۱۰-log serials dilution Asia virus RNA that defined ۱۰۱.۲ TCID۵۰/ml. Also, to assess the specificity of primer pairs related to ۳D segment and specific probe was used Swine vesicular disease virus (SVDV) and bluetongue virus (BTV) isolates RNA. The results showed that rRT-PCR is seen as a powerful and valuable tool concomitant with routine diagnostic methods for FMD virus diagnosis.

Keywords:

Real , time RT , PCR , Conventional RT , Foot , and , mouth disease virus