Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
Publish place: Archives of Razi Institute journal، Vol: 59، Issue: 2
Publish Year: 1384
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ARCHRAZI-59-2_008
تاریخ نمایه سازی: 6 دی 1402
Abstract:
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR ۸۰۱ strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined ۰.۱μg/ml, ۱:۳۰۰۰ and ۱:۱۰۰, respectively. The optical densities (OD) were compared with a commercial ELISA kit. Also, the results of ELISA were compared to hemagglutination inhibition (HI) test. The correlation coefficient of the results of both ELISAs and HI was significant (P
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