Extraction, Fractionation, Phytochemical Profile, Antioxidant, and Antimicrobial Activities of Cassia absus L. and Citrus medica L.
Publish place: Pharmaceutical and Biomedical Research، Vol: 9، Issue: 2
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_PBRE-9-2_005
تاریخ نمایه سازی: 10 دی 1402
Abstract:
Background: Cassia absus L. and Citrus medica L. plants are sources of different phytochemicals with pharmaceutical significance. They are biochemically distinctive and used in producing various herbal products with exclusive bioactivity. Also, the extraction of these phytonutrients with medicinal characteristics has recently increased.
Objectives: We determined to evaluate the plant-derived compounds such as polyphenols and antioxidant and antimicrobial activity of the methanolic extract of Cassia absus L. and Citrus medica L.
Methods: Antioxidant activity was calculated through linoleic acid inhibition, determination of reducing power, and ۲,۲-diphenyl-۱-picrylhydrazyl (DPPH) scavenging assay. The disk diffusion method was used for assessing antimicrobial activity. Various solvents such as acetic acid, ethanol, acetone, n-hexane, and ethyl acetate were used to perform partition fractionation of the methanolic extract of plants.
Results: Three major components were analyzed through high-performance liquid chromatography in C. absus and C. medica. Total phenolic contents varied from ۵.۵۸-۱۷.۱ to ۲.۶۶-۷.۴۱ per ۱۰۰ g examined through Folin-Ciocalteu reagent, pointed as gallic acid equivalents (GAE), and total flavonoid contents lined from ۱۱.۵۰-۳۲.۴ to ۲۴.۹۱-۳۶.۳۶ per ۱۰۰ g specified as catechin equivalents. Inhibition rate and reducing potential were greater in C. medica fractions of ۴۹.۶% and ۱.۱۴, respectively. The C. medica solvent fractions exhibited greater radical scavenging activity in the DPPH assay at ۸۰%. C. absus and C. medica extract significantly inhibit different gram-negative (Escherichia coli, Pasteurella multocida, and Fusarium) and gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, Aspergillus flavus, Aspergillus niger, and Fusarium Solani) bacteria.
Conclusion: Both leaf extracts of C. absus and C. medica could have potential applications in agriculture, pharmaceutics, and medicine.
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Authors
Zartasha Kousar
Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.
Surryia Manzoor
Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.
Saima Naz
Division of Science and Technology, University of Education Lahore, Multan Campus ۶۰۷۰۰, Pakistan.
Muhammad Shafiq Shahid
Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khoud ۱۲۳, Muscat, Oman.
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