Evaluation of Fis-۱ and miR-۴۸۴ Expression Levels in Tumor Tissue Samples and Healthy Tumor Margins in Lung Cancer

Background: MicroRNAs (miRNAs) regulate gene expression and various cellular activities. They also hold significant importance in the progression and development of human malignancies. Among these, miRNA-۴۸۴ and the Fis-۱ gene have been identified as having substantial roles in lung cancer. This study aims to ascertain miRNA-۴۸۴ and Fis-۱ gene expression levels in non-small cell lung cancer (NSCLC) patients.Method: In this case-control study, ۴۵ pairs of tumor tissues and their corresponding healthy margin tissues were surgically obtained from NSCLC patients and promptly preserved in liquid nitrogen after excision. Total RNA extraction was performed using TRIzol, followed by cDNA synthesis using a designated kit. Afterward, we used quantitative reverse transcription polymerase chain reaction (qRT-PCR) to measure the expression levels of miRNA-۴۸۴ and the Fis-۱ gene. Furthermore, the clinicopathological characteristics of the NSCLC patients were assessed.Results: Our findings revealed an upregulation of miRNA-۴۸۴ expression and downregulation of Fis-۱ gene expression in NSCLC tissues compared with non-tumor tissues. Additionally, significant correlations were observed between miRNA-۴۸۴ and Fis-۱ gene expression levels and clinicopathological features of the patients, including factors such as lymph node involvement and distant metastasis.Conclusion: These findings suggest the potential utility of Fis-۱ and miR-۴۸۴ as prognostic and diagnostic markers in NSCLC.

Keywords:

miRNA-۴۸۶ , Fis-۱ Protein , Carcinoma , Non-small-cell lung , Malignancy