The phylogenetic reconstruction of biosynthesizing genes from Cyanobacteria isolated from Ziarat waterfall of Golestan Province
Publish place: The Iranian Journal of Botany، Vol: 29، Issue: 2
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJB-29-2_010
تاریخ نمایه سازی: 16 دی 1402
Abstract:
Cyanobacteria are anaerobic and photosynthesizing prokaryotes. In the past, the classification of cyanobacteria was only based on morphological characteristics. Today, advanced techniques such as molecular markers are used to achieve more accurate and reliable classification. In this research, sampling was done from the Ziarat waterfall in Golestan province. After cultivation and purification in solid culture medium BG-۱۱, molecular identification of natural compounds’ biosynthesizing genes (hassallidins synthetase (hasN) and geosmin A (geoA) along with divergence analyses were performed using the amplification of the chloroplast genes RNA polymerase C۱ (rpoC۱).Then, the strain containing the above genes was identified by amplification of ۱۶S rRNA and internal transcribed spacer (ITS) genes. Phylogenetic trees were built using the Maximum Likelihood method and the appropriate model with the help of a web server on the IQ-Tree server. The secondary structure of ITS was drawn in different parts of helix D۱-D۱′, D۲, D۳, tRNAIle, tRNAAla, BOX B, BOX A, and V۳ using the Mfold program. The results showed that only the Nodosilinea sp. ۱۳۵۹ (Leptolyngbyaceae, Synechococcales) strain has the genes mentioned above. In addition, the results of calculating the KA/KS of hasN and geoA genes and the phylogenetic incongruence of ۱۶S rRNA and rpoC۱ genes showed that natural selection by creating positive mutations has led to diversity in the studied strain. This study is among the first research conducted on the molecular phylogeny of cyanobacteria producing natural compounds in Ziarat waterfall.
Keywords:
Nodosilinea sp. ۱۳۵۹ , ۱۶S rRNA , ITS , hasN , geoA , rpoC۱ , biosynthesizing genes , Natural compounds
Authors
Bahareh Nowruzi
Department of Biotechnology, Faculty of Converging Sciences and Technologies, Islamic Azad University, Science and Research, Tehran, Iran
Samaneh Jafari Porzani
Department of Biotechnology, Faculty of Converging Sciences and Technologies, Islamic Azad University, Science and Research, Tehran, Iran
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