Can Heterogenic Patterns of JAK۲, MPL, and CALR Genes Predict Specific Clinical Characteristics of Myeloproliferative Disorders?
Publish place: Personalized Medicine Journal، Vol: 8، Issue: 31
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
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JR_PMJ-8-31_001
تاریخ نمایه سازی: 19 دی 1402
Abstract:
Myeloproliferative neoplasm (MPN) is a neoplasm with three categories; essential thrombocythemia (ET), polycythemia vera (PV), and primary myelofibrosis (PMF) and it usually is diagnosed through mutation analysis in several essential genes; JAK۲, MPL, CALR. The mutations of mentioned genes in ۵۰ patients with MPN and ۵۰ healthy volunteers were determined via allele-specific PCR and sequencing. Based on the results, MPN and its subtypes have significant relation with mutations (p<۰.۰۵). JAK۲ (exon ۱۴) mutation was related to MPN and its subtypes except for ET and CALR (exon ۹) type ۱ was merely related to ET, but CALR (exon ۹) type ۲ mutation was more prevalent in MPN and PV (p<۰.۰۵). None of the mutations co-occurred simultaneously. There was no evidence of mutation in JAK۲ (exon ۱۲) and MPL (exon ۹ and ۱۰) in our study, so they are unsuitable diagnostic candidates. So, mutations in JAK۲ (exon ۱۴), and CALR (exon ۹) type ۱ and ۲ are essential in MPN diagnosis in Iranians.
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Authors
Measam Morsali
Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Mahdieh Mehrab Mohseni
Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Maryam Naseroleslami
Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.