Effect of Helichrysum plicatum Subs. plicatum on Fructose-induced Nonalcoholıc Fatty Liver

Background: The Helichrysum genus of the Asteraceae family comprises ۵۰۰ species. Fifteen Helichrysum species are found in Turkey. This genus is used in traditional medicine for several beneficial effects.Objectives: This study explored Helichrysum plicatum subsp. plicatum (HPsP) effects on fructose-induced nonalcoholic fatty liver disease (NAFLD). Methods: Thirty-five adult male Wistar rats weighing ۲۰۰–۲۵۰ g were divided into ۵ groups: Control, HPsP, fructose (F), HPsP+fructose, and double-dose HPsP+fructose. Fructose (۴۰%) was then added to the water. HPsP extract was administered at doses of ۴ and ۸ mg/kg. All treatments were performed for ۸ weeks.Results: The F, F+HPsP, and double-dose groups exhibited higher palmitic acid (۱۶:۰) levels than the control group (P<۰.۰۰۱). F+HPsP treatment enhanced palmitoleic acid levels (P<۰.۰۰۱). The F and F+HPsP groups showed reduced levels of linolenic (۱۸:۳ n-۳), eicosadienoic (۲۰:۲, n-۶), and arachidonic (۲۰:۴ n-۶) acids (P<۰.۰۰۱, P<۰.۰۱, and P<۰.۰۵, respectively). The F, F+HPsP, and double-dose groups showed lower docosahexaenoic acid (۲۲:۶, n-۳) levels (P<۰.۰۵). Retinol and in HPsP treated group and (P<۰.۰۵) K۱ vitamin levels increased in all groups compared to control group (P<۰.۰۰۱). The F, F+HPsP, and HPsP groups had lower vitamin K-۲ and D-۳ levels (P<۰.۰۰۱ and P<۰.۰۱, respectively). The F, HPsP, F+HPsP, and double-dose groups had higher cholesterol levels (P<۰.۰۵, P<۰.۰۱, P<۰.۰۰۱, respectively).Conclusion: HPsP extract altered fructose-induced fatty liver composition and levels of reduced glutathione and vitamins A, D, E, and K. Unexpectedly, high liver cholesterol levels require treatment.

Keywords:

Fructose , Helichrysum plicatum , Lipophilic vitamins , NAFLD , Omega-۳