Basil and Dracocephalum kotschyi alcoholic extracts affect BCL۲ expression and HepG۲ cell proliferation

Background: Liver cancer is the second most common reason for cancer-related death globally, and thus, a major public health challenge. Plant-based drugs are promising therapeutic agents against cancers. Basil and Dracocephalum kotschyi extracts also exhibit therapeutic impact on various cancers. To assess the effects of the extract of Basil and Dracocephalum kotschyi on cell proliferation and BCL۲ expression in the HepG۲ cell line. Materials and methods: IIn this experimental study, the HepG۲ cell line was selected for treatment with the alcoholic extracts of Basil and Dracocephalum kotschyi. Cell proliferation was examined in the presence of various concentrations of the Basil and Dracocephalum kotschyi extracts by MTT assay. Moreover, the effect of the alcoholic extracts of Basil and Dracocephalum kotschyi on BCL۲ expression was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Results: Cell proliferation analyses showed anticancer characteristics of the alcoholic extracts of Basil and Dracocephalum kotschyi. In addition, treatment with the extracts of Basil and Dracocephalum kotschyi led to the decreased expression of BCL۲ in HepG۲ cell line. Conclusion: These findings indicated that Basil and Dracocephalum kotschyi are promising candidates for future anticancer research.

Keywords:

Basil , BCL۲ , Dracocephalum kotschyi , HepG۲ , Liver cancer.