Protective effects of naringin against oxaliplatin-induced testicular damage in rats: Involvement of oxidative stress, inflammation, endoplasmic reticulum stress, apoptosis, and histopathology
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 27، Issue: 4
Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
View: 80
This Paper With 9 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-27-4_009
تاریخ نمایه سازی: 14 بهمن 1402
Abstract:
Objective(s): Oxaliplatin (OXL) is a platinum-based chemotherapeutic agent widely used in the treatment of colorectal cancer. Unfortunately, this important drug also causes unwanted side effects such as neuropathy, ototoxicity, and testicular toxicity. This study aimed to investigate the possible protective effects of naringin (NRG) against OXL-induced testicular toxicity in rats. Materials and Methods: TIn the present study, rats were injected with OXL (۴ mg/kg, b.w./day, IP) in ۵% dextrose solution ۳۰ min after oral administration of NRG (۵۰ and ۱۰۰ mg/kg, b.w./day) on the ۱st, ۲nd, ۵th, and ۶th days. Then, the rats were sacrificed on the ۷th day and the testicular tissues were removed. Results: The results showed that NRG decreased (P<۰.۰۰۱) lipid peroxidation, increased (P<۰.۰۰۱) the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and the levels of glutathione (GSH), and also maintained the testis histological architecture and integrity. NRG decreased the levels of apoptosis-related markers such as caspase-۳, Bax, and Apaf-۱ and increased Bcl۲ in the OXL-induced testicular toxicity (P<۰.۰۰۱). In addition, NRG reversed the changes in mRNA transcript levels of oxidative stress, inflammation, and endoplasmic reticulum stress parameters such as Nrf۲, HO-۱, NQO۱, RAGE, NLRP۳, MAPK-۱۴, STAT۳, NF-κB, IL-۱β, TNF-α, PERK, IRE۱, ATF۶, and GRP۷۸ in OXL-induced testicular toxicity (P<۰.۰۰۱). Conclusion: Our results demonstrated that NRG can protect against OXL-induced testicular toxicity by enhancing the anti-oxidant defense system and suppressing apoptosis, inflammation, and endoplasmic reticulum stress.
Keywords:
Apoptosis , Endoplasmic reticulum - stress , Inflammation , Naringin , Oxaliplatin , Testicular toxicity
Authors
Nurhan Akaras
Department of Histology and Embryology, Faculty of Medicine, Aksaray University, Aksaray, Turkey
Cihan Gur
Department of Biochemistry, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey
Cuneyt Caglayan
Department of Medical Biochemistry, Faculty of Medicine, Bilecik Seyh Edebali University, Bilecik, Turkey
Fatih Kandemir
Department of Medical Biochemistry, Faculty of Medicine, Aksaray University, Aksaray, Turkey
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :