The effects of hesperetin on apoptosis induction and inhibition of cell proliferation in the prostate cancer PC۳ cells

ntroduction: Prostate cancer is the second leading cause of cancer-related deaths and the mostcommon cancer diagnosed in men in the United States and Europe. Hesperetin, a member of thef lavonoids with antioxidant property, is found in fruits such as oranges and red fruits. This study was undertaken to evaluate the effects of hesperetin on apoptosis induction and inhibition of cell proliferation in the prostate cancer PC۳ cells.Methods: PC۳ cell line was cultured in standard condition. The cells were exposed to differentconcentrations of hesperetin (۰-۱۰۰۰ μM) for ۴۸ hours. Cell viability was measured by MTT assay.Apoptosis induction was assessed by Annexin V-FITC by flow cytometry analysis.Results: The PC۳ cells exposed to hesperetin (۰-۱۰۰۰ μM) exhibited an IC (inhibitoryconcentration of ۵۰%) about ۴۵۰ μM. At different concentrations of hesperetin (۴۰۰, ۴۵۰ and ۵۰۰µm), the apoptosis increased slightly (not significant) in treated PC۳ cells compared to the controlgroup (۵.۴%, ۷.۸% and ۹.۱% respectively vs. ۴.۲%).Conclusion: These results clearly show that hesperetin can lead to inhibition of PC۳ cellsproliferation.