Role of orFD Pseudomonas aeruginosa H۱۰۳ Gene in Glucose Uptake

Background:Pseudomonas aeruginosa is a gram negative non facultative bacterium and one of the members of normal flora in different sites of body in healthy humans.this bacterium can resist in fluids and hospital environments for a long time.Pseudomonas aeruginosa has two systems for glucose uptake:a low affinity oxidative pathway and a high affinity phosohorylative pathway.Although the role of these genes are unknown by now,they may have a role in regulation of glucose uptake.In the present study,the role of orFD gene in glucose uptake in P.aeruginosa has been investigated.   Method:orFD fragment were cloned in pUCP۲۰ as vector and the recombinant plasmid transferred into WMA۲۰۰ strain of P.aeruginose,a mutant strain of p.aeruginosa with a chromosomal deletion of orFBCD.So we compared the rats of glucose uptake by p.aeruginosa by using labeled glucose under condifions at low substrate concentration and low cell density.   Results:Carbohydrate uptake patterns differed considerably among three strains.the wild type is able to uptake glucose at a faster rate than the mutant:however,the mutant complemented with orFD shows an intermediate uptake comparing to the wild type and the mutant.   Conclusion:orFD gene has an important role in carbohydrate uptake in p.aeruhinosa strains however fuether studies are required to determine the involved mechanism.

Keywords:

aeruginosa , Glucose Pseudomonas uptake , OrFB , OrFC , OrFD