Study of the prevalence of Gardnerella vaginalis isolated from vaginal discharges of patients referred to outpatient clinic of Taleghani hospital in Tehran

In this study in order to determine prevalence of Gardnerella vaginalis and sensitivity of the organism to antimicrobial agents specimens were treated in  the outpatient clinic of Taleghani hospital in Tehran. Specimens were considered positive for bacterial vaginosis (BV) which were positive three times out of four. The tests include; finding clue cell, amine  test, homogenesity of this discharges, and PH. To isolate the bacteria, discharges were cultured on human blood bilayer twin ۸۰ ( HBT), the isolated strains were tested for antibiotic sensitivity. The results indicated that ۶۷.۵℅ of the patients had bacterial vaginosis. Based on the results of culturing the isolated colonies, and the results of the biochemical conformational tests, ۲۳.۸℅ of the patients were infected with Gardnerella vaginalis, out of these ۸۲℅ were in people who had bacterial vaginosis. No significant correlation was found between the presence of Gardnerella vaginalis and number  labour's and abortions, however there was a significant correlation with the application of IUD among the subjects. Study of the stained smears made from the discharges, indicated that there was a significant correlation between reduction of lactobacilli population, elevation in the number of gram variable bacteria, and the presence of Gardnerella  vaginalis ( p<۰.۰۵). The results obtained from antibiotic  sensitivity tests indicated that the isolated strains of Gardnerella vaginalis was sensitive to ampicillin (۱۰ mg), sulfomethoxazol + trimethoprim (۲۵ mg) , nitrofurantoin (۳۰۰ mg) , metronidazole (۵۰ mg), nitrofurantoin ۳۰۰ mg, chloramphenicol (۳۰ mg) , tetracycline (۳۰ mg), and resistant to metronidazole (۱۰ mg).

Keywords:

Bacterial vaginosis , Gardnerella vaginalis , vaginal discharge , Metronidazole , sensitivity patterns