In vitro anti-Toxoplasma effects and apoptotic induction of queen bee acid (۱۰-hydroxy-۲-decenoic acid) alone and in combination with atovaquone
Publish place: Archives of Razi Institute journal، Vol: 79، Issue: 2
Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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JR_ARCHRAZI-79-2_010
تاریخ نمایه سازی: 18 اردیبهشت 1403
Abstract:
Toxoplasmosis, which is created by the Toxoplasma gondii parasite is a parasitic, infectious disease. ۱۰-hydroxy-۲-decenoic acid (۱۰-H۲DA, queen bee acid (QBA), is one of the most prevalent fatty acid (>۴۰%) presents in royal jelly. Studies reported various beneficial effects of ۱۰-H۲DA antitumor, anti-inflammatory, antiangiogenic, improving the immune system, and antimicrobial effects. This experimental survey aimed to evaluate the in vitro efficacy of QBA against tachyzoites and intracellular parasites of the T. gondii RH strain. Anti-Toxoplasma effects of QBA against tachyzoites were examined by MTT (۳-[۴,۵-dimethylthiazol-۲-yl]-۲,۵ diphenyl tetrazolium bromide) assay for ۳۰, ۶۰, ۱۲۰, and ۱۸۰ min. In addition, the effect of QBA on infection rate and intracellular parasites was studied. Real-time PCR was also applied to assess the expression level of the Caspase-۳ gene. The best efficiency of QBA was reported at ۱۰۰ and ۵۰ µg/mL whereas all tachyzoites were diminished followed by ۱۲۰- and ۱۸۰-min treatment, respectively. We also found that the best repressing efficacy of QBA on the infection rate and the load of parasites into the Vero cells was indicated at ۱۰۰ µg/mL (p<۰.۰۰۱): but, the combination of QBA (۱۲.۵ µg/mL) along with atovaqoune ۳۰ µg/mL displayed the highest effect on the infection rate and the load of parasites into the Vero cells in the infected Vero cells. The expression level of the Caspase-۳ gene was dose-dependently increased after exposure of tachyzoites to QBA; mainly at ½ IC۵۰, and IC۵۰ compared to the normal saline. The achieved findings exhibited the high in vitro potency of QBA especially in combination with atovaqoune against T. gondii RH strain tachyzoites. Although apoptosis induction can be suggested as one of the principle mechanisms; more studies are required to elucidate its accurate mechanisms as well as its efficacy and safety in animal models and clinical settings.
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Authors
Panteha Asgari
Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, North Cyprus
Siavash Pourhossein
Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, North Cyprus