Evaluating the Effects of Enrofloxacin on Angiogenesis Using the Chick Embryo Chorioallantoic Membrane Model

Objective- Because little information was available in the literature about the angiogenic property of enrofloxacin, this investigation was undertaken to evaluate this aspect using an in vivomodel.Design- Experimental study.Animals- Twenty fertile chicken eggs (Ross ۳۰۸) with the average egg-weight of ۵۵ ± ۰.۴ g were randomly divided into experimental and control groups.Procedures- Agar pellets were prepared with enrofloxacin concentrations at a dosage of ۱۰ mg/Kg egg-weight. A window was carved on the eggshell and drug-impregnated pellet applied on day ۸ of incubation on the surface of the chick chorioallantoic membrane. The response of vascular plexus to the drug application was evaluated.Results- Our results showed that enrofloxacin decrease angiogenesis as shown by a reduction in the morphometric parameters of vascular plexus including total vessel's length, the number of vascular branch, vascular complexity and capillary density (p<۰.۰۵).Conclusion and Clinical Relevance- Based on findings, it is suggested that enrofloxacin offer a new class of anti-angiogenic agents that might augment the drugs available for the clinical treatment of angiogenesis related diseases.

Keywords:

Angiogenesis , Chorioallantoic membrane , enrofloxacin