Evaluating the Feasibility of Esophagotomy Suture Line Reinforcement Using Platelet Rich Fibrin Membrane and is Effect on Wound Healing

Objective- This study aimed to evaluate the feasibility of using platelet rich fibrin membrane as a novel on-lay patching biomaterial in canine esophagotomy and its effects on esophageal wound healing.Design- Experimental study.Animals- Eight adult mixed breed dogs of both sexes equally allocated to control and treatment groups.Procedures- Longitudinal incisions measuring ۳ cm were made in the cervical esophagus of all dogs (۲ incisions in each dog). All incisions were sutured and on-lay patching was performed in four dogs using platelet rich fibrin. After ۲۸ days, wound healing was assessed by macroscopic, histological and biochemical methodsResults- Patching resulted in less adhesion formation (۱۸.۲۴ vs. ۶۱.۶۷ mm, p<۰.۰۵) and increase in tissue hydroxyproline content (۹۱.۳۱ vs. ۷۴.۳۱ mg, p>۰.۰۵). Histologically, platelet rich fibrin membrane mostly influenced wound healing in the outer layers of the esophagus particularly the muscular layer although a slightly better wound healing was observed overall.Conclusion and Clinical Relevance- Platelet rich fibrin membrane could be used as an alternative patching biomaterial in esophageal surgery although further investigations needs to be carried out particularly in clinical cases.

Keywords:

Platelet rich fibrin membrane , Onlay patching , esophagus , Dog , wound healing