Benidipine Reduces Ischemia/Reperfusion Injury Following Testicular Torsion/Detorsion in Rats

Objective-This study aimed to determine the protective effects of benidipine on testicular injury after testis torsion/detorsion in ratsDesign-Experimental studyAnimals-Fifteen male Sprague-Dawly rats weighing ۲۰۰-۲۲۰gProcedure-Rats were randomly divided into ۳ groups: sham (healthy group with sham operation, n=۵), IRC group (control group in which I/R injury was performed by torsion the right testis ۷۲۰ º clockwise for ۲ hours and detorsion for ۲ hours, n=۵), and IRB group (IR + benidipine, I/R process was performed following orally benidipine administration + ۴ mg/kg benidipine, n=۵). Levels of glutathione peroxidase (GPx) and malondialdehyde (MDA) were determined in testicular tissues of rats. Testicular tissues were also examined histopathologically. Results-The levels of MDA (P<۰.۰۰۰۱) was significantly increased augmented in the testis of IRC group rat. Testicular GPx levels were significantly reduced (p<۰.۰۰۰۱) after I/R. Administration of benidipine before torsion prevented the increase in lipid peroxidation and alleviated GPx levels. Benidipine also impeded ischemia/reperfusion cellular damage and histological alternations in testicular tissueConclusion and clinical relevance-These results show that therapy with benidipine before torsion may induce protective effects against ischemia/reperfusion injury

Keywords:

Benidipine , ischemic – reperfusion , testicular torsion , Rat