The effects of culture media and various NAA, sucrose and mesos concentrations on in vitro propagation of Gerbera jamesonii

Gerbera jamesonii Bolus is one of the most popular ornamental plants worldwide and its micrpropgation allows to produce a large number of true to type plants with good quality in a short time. In vitro propagation of gerbera requires culture media supplemented with specific concentrations of minerals, organic supplements and energy sources. The aim of this study was to determine suitable culture medium and evaluate different concentrations of sucrose and Naphtalic Acetic Acid (NAA) for in vitro development of gerbera explants. Micro shoots of two gerbera cultivars (Artist and Brilliance) were cultured on four different culture media and the effect of various concentrations of sucrose (۲۰ and ۳۰ g L-۱) as well as MgSO۴.۷H۲O and CaCl۲.۲H۲O (۰.۵X, ۱X and ۱.۵X) on in vitro propagation properties were examined. MS medium provided better shoot development and application of lower concentration of sucrose improved the efficiency of gerbera micropropagation. In Artist cultivar, the highest number of shoots was obtained by using ۲۰ g L-۱ of sucrose and with or without NAA application (۹.۰۸ micro shoots per explant). In contrast, Brilliance produced the highest number of shoots by using ۲۰ g L-۱ of sucrose and ۰.۱ mg L-۱ of NAA (۷.۴ micro shoots per explant). In addition, the increase or decrease of MgSO۴.۷H۲O and CaCl۲.۲H۲O did not change propagation efficiency. The results can contribute to optimize bioreactor systems for large-scale production.

Keywords:

Gerbera jamesonii , carbon source , growth regulator , micropropagation