Evaluation of tap water quality and molecular identification of Escherichia coli O۱۵۷ in Al-Kut City, Iraq
Publish place: Caspian Journal of Enviromental Sciences، Vol: 20، Issue: 5
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_CJES-20-5_020
تاریخ نمایه سازی: 31 خرداد 1403
Abstract:
Escherichia coli is a pathogen which can result in gastrointestinal infection. It can be transferred in a many methods, like water and food. This study is aimed to evaluate the potable water quality in Al- Kut City, Iraq, in addition to diagnosis of E. coli O۱۵۷:H۷ by PCR protocol. The method includes examination for total dissolved solids (TDSs), pH and electrical conductivity (EC), as well as the bacterial DNA extraction by DNA extraction kit, then detection via PCR. Primer "Rfb" was used for detection of O۱۵۷ gene. The bands of PCR product was ۲۹۲ bp, which has been visualized using gel electrophoresis. The results gained revealed that the advanced procedure by molecular detection may be used as a routine examination for drinking water pollution. Moreover, it is a precise procedure providing results at a less time compared to the classical culture methods.
Authors
Malath I. Yousif
Al-Furat Al-Awsat technical university, Technical Institute, Diwanya , Iraq
Safa Hasan Radhi
Department of Biology, College of Science, Al-Qasim Green University, Iraq
Shaimaa Awadh Auda
College of dentistry, Al-Muthanna University, Iraq
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