Interleukin-۱۰ Gene Polymorphisms Modulate the Risk of Infertility in Chlamydia trachomatis Positive Kurdish Women in Erbil Province

Background: There is evident inter-individual variability in women's responses to Chlamydial infections and reproductive tract problems. Women's genetic variations within the Interleukin-۱۰ (IL-۱۰) gene have been linked to variances in response to Chlamydia trachomatis infection. This study was aimed to demonstrate the profound association of IL-۱۰ with infertility and demonstrate the role of IL-۱۰ (-۵۹۲ C/A rs۱۸۰۰۸۷۲) and (-۱۰۸۲ A>G rs۱۸۰۰۸۹۶) single nucleotide polymorphism (SNPs) gene in the susceptibility and severity of a C. trachomatis infection. Method: In this evaluation study, serum IL-۱۰ concentration was measured in ۱۳۴ women diagnosed with infertility and ۵۰ healthy volunteers by enzyme-linked immunosorbent assay (ELISA). The tetra-amplification refractory mutation system-PCR (T-ARMS-PCR) analysis was performed to detect the genotyping of the rs۱۸۰۰۸۷۲ and rs۱۸۰۰۸۹۶ SNPs genes. Result: Both female groups were positive for anti-chlamydial IgM antibody, but the intensity of response differed between cases. At the same time, the incidence of genital C. trachomatis by PCR was ۴۶.۲% in infertile women. The serum concentration of IL۱۰ was lower in infertile women than healthy participants and higher in infertile C. trachomatis-positive women compared to infertile C. trachomatis-negative in all groups except endometriosis (Endo) infertility. In rs۱۸۰۰۸۷۲, the CA genotype and C allele are associated with an increased risk for infertility, except in polycystic ovarian syndrome (PCOS), which is an A allele. In the case of rs۱۸۰۰۸۹۶, the AG genotype and G allele show a greater risk for infertility. Conclusions: Our results confirmed that rs۱۸۰۰۸۷۲ and rs۱۸۰۰۸۹۶ gene polymorphisms were associated with an increased risk of C. trachomatis infection.

Keywords:

Chlamydia trachomatis , Female infertility , IL-۱۰ , SNP