The Potential Association Between microRNA ۱۳۵-۵P and p۶۲ and Their Effect on NRF۲ Pathway in Multiple Sclerosis

Background: Multiple Sclerosis (MS) is a prevalent non-traumatic disabling disease affecting young adults, characterized by complexity in its pathogenesis. Nuclear factor erythroid ۲-Related Factor ۲ (NRF۲) serves as a crucial transcriptional regulator of anti-inflammatory and antioxidant enzymes, influenced by the ubiquitous protein p۶۲. It acts as a scaffold directing substrates to autophagosomes. This study aims to explore the potential association between microRNA ۱۳۵-۵p and p۶۲ and their impact on inflammation and oxidative stress through the NRF۲ pathway in MS. Methods: The study included ۳۰ healthy controls and ۶۰ MS patients (relapsing-remitting and secondary progressive). Real-time PCR was employed for the detection of Nrf۲, p۶۲, miRNA۱۳۵-۵P, and NF-κB in serum, while p۵۳ levels were determined using ELISA. Results: Nrf۲ and p۶۲ expression was significantly downregulated in the MS group compared to controls. Conversely, miRNA۱۳۵-۵P, NF-κB expression, and P۵۳ levels were significantly elevated in the MS group. Conclusion: This study reveals a potential association between miRNA ۱۳۵-۵p and p۶۲, indicating their role in the pathogenesis of MS. Results suggest that miRNA ۱۳۵-۵p and p۶۲ may influence inflammation and oxidative stress in MS through the NRF۲ pathway, potentially mediated by NF-κB and p۵۳.

Keywords:

p۶۲ , Microrna۱۳۵ , Multiple sclerosis , NRF۲.