Association of Mir-۱۴۹C/T (rs۲۲۹۲۸۳۲) Polymorphism and Lung Carcinoma Occurrence in the Iranian Population: A Case-Control Study

Introduction: Lung carcinoma (LC) is a group of anomalies associated with abnormal respiratory cell divisions. Some studies reported the possible role of the miR-۴۹۹ (rs۳۷۴۶۴۴۴) polymorphism in LCs in various societies. In the current case-control study, we decided to investigate the role of miR-۱۴۹C/T variant on LC occurrence in the Iranian population.Materials and Methods: In this case-control study, a total of ۱۷۲ subjects (۷۲ LC patients and ۱۰۰ control) were genotyped by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique, for this aim, ۵ ml peripheral blood sample was obtained from each subject and the genomic DNA was extracted by the salting-out method. After genotyping and data collection, the frequency of alleles and genotypes were statistically analyzed (SPSS, v.۲۰).Results: Since the results showed, in the frequency of all types of genotypes (Dominant, Codominant, Over-dominant, and Recessive) and associated alleles (C and T) in miR۱۴۹C/T no significant difference (p >۰.۰۵) was observed in comparison between the patient group and the control group.Conclusions: According to the findings of this study there is no significant relation between the miR-۴۹۹C/T (rs۳۷۴۶۴۴۴) polymorphism and lung cancer occurrence and this polymorphism is not a significant risk factor for lung cancer in the Iranian population.

Keywords:

Carcinoma , Lung , , rs۲۲۹۲۸۳۲ , miR-۱۴۹C/T , Polymorphism