A New Xeno-free Medium for Human Mesenchymal Stem Cell Expansion ex vivo

Introduction: An optimal culture medium that can rapidly and efficiently proliferate cells ex vivo is very crucial for developing mesenchymal stem cells (MSCs)-based tissue engineering and regenerative medicine. We developed a set of MSCs ex vivo proliferation medium, mscGOTM XF, which consists of a basal medium and a screened human platelet lysate.Materials and Methods: In this study, the developed mscGOTM XF medium was prepared, and then testified by human MSCs isolated from bone marrow, umbilical cord, and fat tissue. The proliferation, surface markers, differentiation, and chromosomal stability of MSCs cultured in mscGOTM XF medium were investigated.Results: The mscGOTM XF medium could sustain MSCs at a high proliferation rate, with the population doubling time of ۱۶ to ۳۹ hours (depending on the type and passage number of MSCs). The proliferated MSCs could express CD۱۰۵, CD۹۰, and CD۷۳, lack expression of CD۳۴ and CD۴۵; and maintain the capacity to differentiate into adipocytes, osteoblasts, and chondrocytes. Additionally, G-Band karyotyping data confirmed chromosome stability in the duration of cell culture at passage ۵ and passage ۷.Conclusions: The mscGOTM XF medium could sustain MSCs proliferation ex vivo and exhibit the potential to be developed into a clinical-grade cell culture medium kit.

Keywords:

Xeno-free Medium , Mesenchymal Stem Cells , Cell proliferation , cell differentiation