Kisspeptin Heterologous Expression Affects the Growth of Recombinant Escherichia coli Cells

Kisspeptin peptides are the products of kiss۱ gene that offer their activities via G protein-coupled receptor known as GPR۵۴. The GPR۵۴-Kiss۱ pathway has an essential role in initiation and controlling the mammalian fertility. This interactive pathway affects the fertility of patients with polycystic ovary syndrome (PCOS). To increase our knowledge, this study was aimed to analyze the molecular interaction and the heterologous expression of an amplified mutated form of kisspeptin from PCOS patient. The molecular interaction of an amplified Kiss۱ exon۳ product with GPR۵۴ receptor was analyzed by molecular docking computational method. Using His tag-based recombinant technology, the heterologous expression and production of mutated kisspeptin was examined and reported in Escherichia coli cells. Kisspeptin product was predicted to be strongly interacted to GPR۵۴ receptor molecule with a binding energy of ۲۲۹.۴۵ kcal/mol and key intermolecular interactions including hydrogen bonding and hydrophobic attraction. The results related to expression analysis showed that the growth of recombinant bacteria is considerably affected by about ۴۸% as compare to non-recombinants. The high affinity interactive pattern between mutated kisspeptin, receptor protein, and its inhibitory effect on cell growth as novel results may affect the physiology and etiology of fertility and PCOS conditions that needs to be identified by further investigations.

Keywords:

GPR۵۴ , KISS۱ , polycystic ovary , Receptor