Comparison of the Antimicrobial Efficacy of Calcium Hydroxide and Photodynamic Therapy Against Enterococcus faecalis and Candida albicans in Teeth With Periapical Lesions; An In Vivo Study

Abstract Introduction: Elimination of pathological microflora of root canal systems is a major goal in endodontic treatment. This study aimed to compare the antimicrobial efficacy of calcium hydroxide as an intracanal medication and antibacterial photodynamic therapy (aPDT) against Enterococcus faecalis and Candida albicans in teeth with periapical (PA) lesions.Methods: This in vivo study was conducted on ۲۰ patients with single-rooted mandibular premolar with previously failed endodontic treatment. This study was performed as a clinical trial (IRCTID: IRCT۲۰۱۶۰۹۰۴۲۹۶۸۶N۱). After conventional chemo-mechanical root canal preparation (hand and rotary instruments and ۲.۵% NaOCl), microbiological samples were obtained using sterile paper points, then stored in thioglycolate solution and transferred to a microbiology laboratory. Group ۱ (n = ۱۰) specimens underwent aPDT (diode laser ۸۰۸ nm + ۵۰ mg/mL methylene blue), while creamy calcium hydroxide paste was used in group ۲ for a duration of ۱ week. A control sample was taken with sterile paper points and F۳ Protaper rotary file. The samples were dispersed in transport medium, serially diluted, and cultured on selective mediums to determine the number of colony forming units (CFUs). Data were analyzed by Mann-Whitney U test at ۵% significance level. The significance level for all analyses was set at P<۰.۰۵.Results: Number of CFU significantly decreased in both groups after the interventions (P<۰.۰۰۱); however, there was no significant difference in the colony count between the ۲ groups.Conclusion: aPDT and calcium hydroxide therapy showed the same antimicrobial efficacy on E. faecalis and C. albicans. Keywords: Antimicrobial efficacy Calcium hydroxide Enterococcus faecalis Photodynamic therapy Root canal treatment