In Vitro Study of Anti-Inflammatory and Anti-Oxidant Activity of Four Gingers with Various Solvent Extractions

Backgrounds: Ginger research could lead to the discovery of its potential benefits for various applications, including medicinal therapy, nutrition, and pharmacology. This study aimed to analyze the anti-inflammatory and anti-oxidant activities of four ginger extracts using different polar and non-polar solvents. Materials & Methods: The dry powder (۵۰ g) of four gingers was macerated using acetone, dimethyl sulfoxide, ethanol, methanol, chloroform, hexane, petroleum ether, and ethyl acetate and then placed on a rotating shaker (۲۵ rpm) at ۲۸ ℃ for ۵ days until extraction. The prepared extracts underwent biological screening assays for anti-oxidant and anti-inflammatory properties. Findings: The acetone extract of Alpinia calcarata had the lowest IC۵۰ value for DPPH scavenging. The IC۵۰ value of A. calcarata dimethyl sulfoxide extract was ۸۹.۵۹ µg/mL for ABTS activity. The IC۵۰ value of A. calcarata acetone extract for hydrogen peroxide scavenging was ۸۵.۴۳ µg/mL. Evaluation of the anti-inflammatory potential of ginger extracts showed that the petroleum ether extract of Zingiber zerumbet had the lowest IC۵۰ value of ۹۰.۹۵ g/mL for albumin stabilization compared to the other solvents. Conclusion: The study compared solvent extracts of different gingers, revealing diverse bioactivity depending on the extraction method. A. calcarata and Z. zerumbet extracts showed strong antioxidant and anti-inflammatory properties. These extracts had lower IC۵۰ values, demonstrating their efficacy in blocking proteinases, stabilizing cellular components, and scavenging free radicals. These results provide valuable insights into the pharmacological potential of these extracts as natural antioxidants and anti-inflammatory agents.

Keywords:

Rhizomatous plants , Zingiberaceae family , Polar and non-polar extraction , Anti-oxidant and anti-inflammatory activities