Molecular survey and phylogenetic analysis of Bartonella henselae and Bartonella clarridgeiae in dogs from northwest Iran
Publish place: Journal of Zoonotic Disease، Vol: 8، Issue: 4
Publish Year: 1403
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JZD-8-4_004
تاریخ نمایه سازی: 23 شهریور 1403
Abstract:
The purpose of the current study was to examine the occurrence and genetic characteristics of Bartonella henselae (B. henselae) and Bartonella clarridgeiae (B. clarridgeiae) in dogs from West Azerbaijan province, Iran. Blood samples were obtained from ۴۰۰ dogs, and their gender, age, reproductive status, ownership status, and geographical origin were documented. Positive samples were identified using PCR and sequencing techniques, and the gene sequences of the ftsZ (for B.henselae) and gltA (for B.clarridgeiae) genes were examined using BioEdit software. The gene sequences acquired demonstrated a minimum similarity of ۱۰۰.۰۰% when compared to the reference sequences in the GenBank® database. Additionally, a phylogenetic tree was built using MEGA۱۱. The findings of the study indicated that ۸.۵% (p<۰.۰۵; ۹۵%, CI: ۶.۱۵%–۱۱.۶۴%) of the tested dogs were positive for B. henselae, and ۳.۲۵% (p<۰.۰۵; ۹۵%, CI: ۱.۹۱%–۵.۴۸%) were positive for B. clarridgeiae. The results for both Bartonella species showed a significant difference (p۰.۰۰۱) between neutered and non-neutered dogs, as well as a significant difference (p=۰.۰۰۱ and p=۰.۰۰۴) between stray and pet dogs. The study's findings highlight the significant role that dogs could potentially engage as the origins of Bartonella infection, as a zoonotic agent, in the region.
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Authors
Sima Alempour Rajabi
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abdolghaffar Ownagh
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Mojtaba Hadian
Department of Clinical Pathology and Internal Medicine, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
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