An encompassing approach for developing and validating a novel reversed phase UFLC methodology for the concurrent assessment of curcumin, resveratrol, silybin, and mangiferin from hydrogels for transdermal drug delivery

Background and Objectives: In topical preparations, four herbal compounds—mangiferin, resveratrol, silybin, and curcumin—need to be quantified quickly and accurately. Reversed phase Ultra-Fast Liquid Chromatography (UFLC) was used because precise quantification is critical to pharmaceutical research to precisely quantify curcumin, resveratrol, mangiferin, and silybin in topical formulations. Utilizing a Franz diffusion cell setup to evaluate the method's suitability for researching drug content, homogeneity, and percentage release through mice's skin. Methods: A C-۱۸ column (۲۵۰ × ۴.۶mm, ۵µm) was used at ۴۰°C for chromatographic separation. The mobile phase, a gradient of acetonitrile (ACN) and ۰.۱% formic acid in HPLC grade water, was pumped at a rate of ۱ mL per minute. A ۲۰ µL sample was loaded, and analytes were accessed using a photo diode array detector. The method validation included selectivity, linearity (۲-۲۰ µg/mL), accuracy, precision, robustness, and sensitivity. The method's entire run time was ۲۰ minutes. Result: The UFLC method was selective for these compounds, and over the concentration range of ۲–۲۰ µg/mL, the method's linearity was demonstrated with a correlation coefficient > ۰.۹۹. Additionally, it was accurate, precise, robust, and sensitive, with a total run time of ۲۰ minutes. Conclusion: In conclusion, the validated reversed phase UFLC method meets the highlighted need for accurate and quick measurement of mangiferin, resveratrol, silybin, and curcumin in topical formulations. Its effective validation and application in researching drug features using a Franz diffusion cell apparatus make it an important analytical tool in pharmaceutical research, ensuring precise drug content, homogeneity, and release properties in topical formulations.

Keywords:

Drug release , Franz diffusion cell , Transdermal Drug content , Poly-herbals.